Measurement

Part:BBa_I20249

Designed by: Jason Kelly, Karen Wong, Nishant Bhat   Group: Endy Lab   (2007-08-15)

Device used to characterize Ribosome Binding Sites through measurement of GFP expression

Device is based on I2055 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions.

Note: Sequence features are wrong

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 76
    Illegal SpeI site found at 62
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 62
    Illegal NotI site found at 68
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 76
    Illegal SpeI site found at 62
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 76
    Illegal SpeI site found at 62
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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